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Molecular analysis of animal tissues for species identification
Author
Chua, Judy Kim Lan
Supervisor
Tan, Ene Choo
Abstract
In a multi-racial society, the identity of the consumer meat sold has a great impact on the different religions and races. In this study, various molecular techniques such as restriction endonucleases digestion, rDNA PCR, RAPD PCR and protein PAGE were used on 11 different meat samples were analyzed for species identification. The species of meat samples include chicken, duck, pig, sheep, ostrich, cow, buffalo, kangaroo, horse, wild boar and deer. Differentiation between the different species were based on DNA and protein banding patterns. From the results obtained, it seemed that RAPD PCR was the best method for species identification and differentiation. Restriction endonucleases digestion could be used to identify the species origin of animals, but the quality of the DNA used must be pure and flee from contaminants. For rRNA analysis, comparison between the species can only be done on the same gel, since only one major band is visible under UV light. Protein analysis of the species is easier to perform than the rest of the methods, but due to the close proximity of the bands, species identification and differentiation is difficult. Other than for species identification the same techniques can be used the characterization, construction of genetic and physical maps, forensic casework, wildlife conservation as well as for investigating the relationship between two geographically distinct species. In addition to these techniques, other methods such as FINS, immunological assay and hybridization probes are also used for identification of the origin of the species.
Date Issued
1996
Call Number
QH581.2 Chu
Date Submitted
1996