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Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9
Citation
Chen, J. W., Koh, C.-L., Sam, C.-K., Yin, W.-F., & Chan, K.-G. (2013). Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9. Sensors, 13, 13217-13227. http://www.mdpi.com/1424-8220/13/10/13217
Author
Chan, Jian Woon
•
Koh, Chong Lek
•
Sam, Choon Kook
•
Yin, Wai Fong
•
Chan, Kok Gan
Abstract
In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
Publisher
MDPI
Journal
Sensors