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Bioactive natural products from microalgae
Author
Tay, Choon Yen
Supervisor
Tan, Lik Tong
Abstract
The objective of this research project is to discover and identify new bioactive and potential therapeutic agents from the marine cyanobacterium, Lyngbya majuscula, collected from Pulau Hantu Besar, Singapore. The organic extract of this microorganism was obtained and screened for preliminary toxicity using the brine shrimp lethality test. The bioactive secondary metabolites were isolated and purified using various chromatographic techniques such as Vacuum Liquid Chromatography (VLC) and High Performance Liquid Chromatography (HPLC). The bioassay-guided fractionation on the organic extracts and purification led to the isolation of a new compound designated as 120-D. However, this compound, 120-D, was observed to have no toxicity in the brine shrimp lethality tested at 10ppm. Its planar structure was elucidated using Mass Spectrometry and extensive 1D and 2D NMR experiments. A proposal on the absolute stereochemical determination as well as the biosynthesis of compound 120-D are presented.
Date Issued
2008
Call Number
QK568.M52 Tay
Date Submitted
2008