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Title: Characterization of quorum sensing and quorum quenching soil bacteria isolated from Malaysian tropical montane forest
Authors: Chong, Teik Min
Koh, Chong Lek
Sam, Choon Kook
Choo, Yeun Mun
Yin, Wai Fong
Chan, Kok Gan
Keywords: Arthrobacter
Liquid chromatography mass spectrometry (LC-MS)
N-acylhomoserine lactone
N-dodecanoyl-L-homoserine lactone
P-coumaroylhomoserine lactone
Pseudomonas frederiksbergensis
Quorum quenching
Quorum sensing
Rapid resolution liquid chromatography (RRLC)
Issue Date: 2012
Citation: Chong, T. M., Koh, C. L., Sam, C. K., Choo, Y. M., Yin, W. F., & Chan, K. G. (2012). Characterization of quorum sensing and quorum quenching soil bacteria isolated from Malaysian tropical montane forest. Sensors, 12, 4846-4859.
Abstract: We report the production and degradation of quorum sensing N-acyl-homoserine lactones by bacteria isolated from Malaysian montane forest soil. Phylogenetic analysis indicated that these isolates clustered closely to the genera of Arthrobacter, Bacillus and Pseudomonas. Quorum quenching activity was detected in six isolates of these three genera by using a series of bioassays and rapid resolution liquid chromatography analysis. Biosensor screening and high resolution liquid chromatography-mass spectrometry analysis revealed the production of N-dodecanoyl-L-homoserine lactone (C12-HSL) by Pseudomonas frederiksbergensis (isolate BT9). In addition to degradation of a wide range of N-acyl-homoserine lactones, Arthrobacter and Pseudomonas spp. also degraded p-coumaroyl-homoserine lactone. To the best of our knowledge, this is the first documentation of Arthrobacter and Pseudomonas spp. capable of degrading p-coumaroylhomoserine lactone and the production of C12-HSL by P. frederiksbergensis.
ISSN: 1424-8220
Other Identifiers: 10.3390/s120404846
Appears in Collections:Journal Articles

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