Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/15535
Title: 
Degradation of bacterial quorum sensing signaling molecules by the microscopic yeast Trichosporon loubieri isolated from tropical wetland waters
Authors: 
Keywords: 
Basidiomycetous
Biosensor
Lactonase
N-acylhomoserine lactone
Quorum sensing
Quorum quenching
Rapid Resolution Liquid Chromatography
Trichosporon loubieri
Yeast
Issue Date: 
2013
Citation: 
Wong, C. S., Koh, C. L., Sam, C. K., Chen, J. W., Chong, Y. M., Yin, W. F., & Chan, K. G. (2013). Degradation of bacterial quorum sensing signaling molecules by the microscopic yeast Trichosporon loubieri isolated from tropical wetland waters. Sensors, 13, 12943-12957.
Abstract: 
Proteobacteria produce N-acylhomoserine lactones as signaling molecules,which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon,a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed
that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded
AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without
oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that
WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of
N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast.
URI: 
ISSN: 
1424-8220
Other Identifiers: 
10.3390/s131012943
Website: 
Appears in Collections:Journal Articles

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