Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/15535
Title: Degradation of bacterial quorum sensing signaling molecules by the microscopic yeast Trichosporon loubieri isolated from tropical wetland waters
Authors: Wong, Cheng Siang
Koh, Chong Lek
Sam, Choon Kook
Chen, Jian Woon
Chong, Yee Meng
Yin, Wai Fong
Chan, Kok Gan
Keywords: Basidiomycetous
Biosensor
Lactonase
N-acylhomoserine lactone
Quorum sensing
Quorum quenching
Rapid Resolution Liquid Chromatography
Trichosporon loubieri
Yeast
Issue Date: 2013
Citation: Wong, C. S., Koh, C. L., Sam, C. K., Chen, J. W., Chong, Y. M., Yin, W. F., & Chan, K. G. (2013). Degradation of bacterial quorum sensing signaling molecules by the microscopic yeast Trichosporon loubieri isolated from tropical wetland waters. Sensors, 13, 12943-12957.
Abstract: Proteobacteria produce N-acylhomoserine lactones as signaling molecules,which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon,a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast.
URI: http://hdl.handle.net/10497/15535
ISSN: 1424-8220
Other Identifiers: 10.3390/s131012943
Website: http://www.mdpi.com/1424-8220/13/10/12943
Appears in Collections:Journal Articles

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