Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/15536
Title: 
Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9
Authors: 
Keywords: 
CepI/CepR homologs
MALDI-TOF mass spectrometry
N-hexanoylhomoserine
Tropical soil bacteria
Triple quadrupole LC/MS
Issue Date: 
2013
Citation: 
Chen, J. W., Koh, C. L., Sam, C. K., Yin, W. F., & Chan, K. G. (2013). Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9. Sensors, 13, 13217-13227.
Abstract: 
In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a
Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors,
namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and
N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
URI: 
ISSN: 
1424-8220
Other Identifiers: 
10.3390/s131013217
Website: 
Appears in Collections:Journal Articles

Files in This Item:
File Description SizeFormat 
Sensors-13-13217.pdf408.01 kBAdobe PDFView/Open
Show full item record

Page view(s)

29
checked on Dec 18, 2018

Download(s) 50

50
checked on Dec 18, 2018

Altmetric