Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/15536
Title: Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9
Authors: Chan, Jian Woon
Koh, Chong Lek
Sam, Choon Kook
Yin, Wai Fong
Chan, Kok Gan
Keywords: CepI/CepR homologs
MALDI-TOF mass spectrometry
N-hexanoylhomoserine
Tropical soil bacteria
Triple quadrupole LC/MS
Issue Date: 2013
Citation: Chen, J. W., Koh, C. L., Sam, C. K., Yin, W. F., & Chan, K. G. (2013). Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9. Sensors, 13, 13217-13227.
Abstract: In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
URI: http://hdl.handle.net/10497/15536
ISSN: 1424-8220
Other Identifiers: 10.3390/s131013217
Website: http://www.mdpi.com/1424-8220/13/10/13217
Appears in Collections:Journal Articles

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