Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/15547
Title: 
Authors: 
Issue Date: 
2012
Citation: 
Chang, C. Y., Koh, C. L., Sam, C. K., Chan, X. Y., Yin, W. F., & Chan, K. G. (2012). Unusual long-chain N-acyl homoserine lactone production by and presence of quorum quenching activity in bacterial isolates from diseased Tilapia fish. PLoS One, 7(8), e44034.
Abstract: 
Growth-dependent cell-cell communication termed quorum sensing is a key regulatory system in bacteria for controlling gene expression including virulence factors. In this study five potential bacterial pathogens including Bacillus sp. W2.2,
Klebsiella sp. W4.2, Pseudomonas sp. W3 and W3.1 and Serratia sp. W2.3 were isolated from diseased Tilapia fish in Malaysia,
supplied by the leading global fish supplier. Proteolytic activity assays confirmed that with the exception of Klebsiella sp. W4.2, all isolates showed distinct proteolytic activity. Furthermore Bacillus sp. W2.2 and Pseudomonas sp. strains W3 and W3.1 also displayed haemolytic activity. By using high resolution liquid chromatography mass spectrometry, we revealed the presence of unusually long-chain N-(3-oxohexadecanoyl)-homoserine lactone (3-oxo-C16-HSL) from Pseudomonas sp. W3.1 and N-dodecanoyl-homoserine lactone (C12-HSL) from Serratia sp. W2.3, respectively. Interestingly, Pseudomonas sp. W3.1 also produced a wide range of Pseudomonas quinolone signalling (PQS) molecules. Pseudomonas sp. W3 did not show any quorum sensing properties but possessed quorum quenching activity that inactivated AHLs. This study is the first
documentation that shows unusual long-chain AHLs production in Serratia sp. and Pseudomonas sp. isolated from diseased
fish and the latter also produce a wide range of PQS molecules.
URI: 
ISSN: 
1932-6203
Other Identifiers: 
10.1371/journal.pone.0044034
Website: 
Appears in Collections:Journal Articles

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