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Title: Closely-related taxa influence woody species discrimination via DNA barcoding: Evidence from global forest dynamics plots
Authors: Pei, Nancai
Erickson, David L.
Chen, Bufeng
Ge, Xuejun
Mi, Xiangcheng
Swenson, Nathan G.
Zhang, Jin-Long
Jones, Frank A.
Huang, Chun-Lin
Ye, Wanhui
Hao, Zhanqing
Hsieh, Chang-Fu
Lum, Shawn
Bourg, Norman A.
Parker, John D.
Zimmerman, Jess K.
McShea, William J.
Lopez, Ida C.
Sun, I-Fang
Davies, Stuart J.
Ma, Keping
Kress, W. John
Issue Date: 2015
Citation: Pei, N., Erickson, D. L., Chen, B., Ge, X., Mi, X., Swenson, N. G., ... & Kress, W. J. (2015). Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots. Scientific reports, 5, 15127.
Abstract: To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots (FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone) and compared the rates of species discrimination (RSD). We quantified RSD by two DNA barcode combinations (rbcL + matK and rbcL + matK + trnH-psbA) using a monophyly-based method (GARLI). We defined two indexes of closely-related taxa (Gm/Gt and S/G ratios) and correlated these ratios with RSD. The combination of rbcL + matK averagely discriminated 88.65%, 83.84% and 72.51% at the local, regional and global scales, respectively. An additional locus trnH-psbA increased RSD by 2.87%, 1.49% and 3.58% correspondingly. RSD varied along a latitudinal gradient and were negatively correlated with ratios of closely-related taxa. Successes of species discrimination generally depend on scales in global FDPs. We suggested that the combination of rbcL + matK + trnH-psbA is currently applicable for DNA barcoding-based phylogenetic studies on forest communities.
ISSN: 2045-2322
Other Identifiers: 10.1038/srep15127
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