Publication: Biomedical potential of marine invertebrate associated bacteria
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Date
2015
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Abstract
Marine invertebrates, such as sponges and corals, are known to host a diverse range of microbes, including bacteria and fungi. These invertebrate-associated microbes have been shown to be producers of bioactive secondary metabolites. In this project, marine bacteria associated with common sponges, Xestospongia testudinaria, Gelliodes fibulata and Clathria reinwardtii and soft corals, Sarcophyton tenuispiculatum, Lobophytum crassum and Sinularia sp., were isolated using culture-dependent method based on actinomycete-selective media. The extracts of the isolated marine bacterial isolates were tested for biological activity in the brine shrimp lethality assay and MTT assay based on the human MOLT- 4 cell line. In addition, phylogenetic analyses of selected bacterial strains that showed significant activity were performed based on their 16S rDNA gene sequences. Phylogenetic analysis was successfully performed for two strains. From the phylogenetic tree, the nearest relatives were found to be Halomonas shengliensis and Halomonas organivorans.