Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/18719
Title: 
Authors: 
Keywords: 
Blood circulation
Haemostasis
Prothrombin
Thrombin
Thrombosis
Fibrin
Issue Date: 
2015
Citation: 
Hiong, K. C., Tan, X. R., Boo, M. V., Wong, W. P., Chew, S. F., & Ip, Y. K. (2015). Aestivation induces changes in transcription and translation of coagulation factor II and fibrinogen gamma chain in the liver of the African lungfish Protopterus annectens. Journal of Experimental Biology, 218: 3717-3728. http://dx.doi.org/10.1242/jeb.125260
Abstract: 
This study aimed to sequence and characterize two pro-coagulant
genes, coagulation factor II ( f2) and fibrinogen gamma chain ( fgg),
from the liver of the African lungfish Protopterus annectens, and to
determine their hepatic mRNA expression levels during three phases
of aestivation. The protein abundance of F2 and Fgg in the liver and
plasmawas determined by immunoblotting. The results indicated that
F2 and Fgg of P. annectens were phylogenetically closer to those of
amphibians than those of teleosts. Three days of aestivation resulted
in an up-regulation in the hepatic fgg mRNA expression level, while
6 days of aestivation led to a significant increase (3-fold) in the protein
abundance of Fgg in the plasma. Hence, there could be an increase
in the blood-clotting ability in P. annectens during the induction phase
of aestivation. By contrast, the blood-clotting ability in P. annectens
might be reduced in response to decreased blood flow and increased
possibility of thrombosis during the maintenance phase of
aestivation, as 6 months of aestivation led to significant decreases
in mRNA expression levels of f2 and fgg in the liver. There could also
be a decrease in the export of F2 and Fgg from the liver to the plasma
so as to avert thrombosis. Three to 6 days after arousal from 6 months
of aestivation, the protein abundance of F2 and Fgg recovered
partially in the plasma of P. annectens; a complete recovery of the
transcription and translation of f2/F2 in the liver might occur only after
refeeding.
URI: 
ISSN: 
0022-0949 (print)
1477-9145 (online)
Other Identifiers: 
10.1242/jeb.125260
Appears in Collections:Journal Articles

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