Transgenic fish technology

Abstract

The advantages and disadvantages of the commonly used techniques for the study of biodiversity and germplasm of organisms are compared. Among all these advanced techniques available, we have established the technique for Random Amplified Polymorphic DNA (RAPD) markers and optimised the conditions for the study of tropical ornamental fish using zebra fish as a model. Optimization was done with the long and short primers which require different conditions. Some of the primers were shown to be very useful as species markers whereas others were selected as useful markers for DNA fingerprinting. After the biodiversity of the organism is established, DNA microinjection technique will be applied to inject foreign DNA into fertilised eggs. Several genes, including the CAT bacterial genes and some other fish genes will be cloned in different plasmid vectors and injected into the embryos. Expression of these transgenic genes at adult stage will be studied.