Please use this identifier to cite or link to this item: http://hdl.handle.net/10497/22217
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dc.contributor.authorPoo, Jeslyn Shi Tingen
dc.contributor.authorChoo, Celine Yen Lingen
dc.contributor.authorHiong, Kum Chewen
dc.contributor.authorBoo, Mel Veenen
dc.contributor.authorWong, Wai Pengen
dc.contributor.authorChew, Shit Funen
dc.contributor.authorIp, Yuen Kwongen
dc.date.accessioned2020-08-13T03:44:07Z-
dc.date.available2020-08-13T03:44:07Z-
dc.date.issued2020-
dc.identifier10.1007/s00338-020-01898-7-
dc.identifier.citationPoo, J. S. T., Choo, C. Y. L., Hiong, K. C., Boo, M. V., Wong, W. P., Chew, S. F., & Ip, Y. K. (2020). Phototrophic potential and form II ribulose-1,5-bisphosphate carboxylase/oxygenase expression in five organs of the fluted giant clam, Tridacna squamosa. Coral Reefs, 39, 361–374. https://doi.org/10.1007/s00338-020-01898-7en
dc.identifier.issn0722-4028 (print)-
dc.identifier.issn1432-0975 (online)-
dc.identifier.urihttp://hdl.handle.net/10497/22217-
dc.descriptionThis is the final draft, after peer-review, of a manuscript published in Coral Reefs. The published version is available online at https://doi.org/10.1007/s00338-020-01898-7en
dc.description.abstractDespite living in oligotrophic tropical waters, giant clams can grow to large sizes because they live in symbiosis with extracellular phototrophic dinoflagellates (zooxanthellae) and receive photosynthates from them. The physical presence of zooxanthellae in five organs (colorful outer mantle, whitish inner mantle, ctenidium, hepatopancreas and foot muscle) of Tridacna squamosa had been confirmed by microscopy, and high densities of zooxanthellae were detected in specific regions of the inner mantle and foot muscle. Symbiotic dinoflagellates use form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) to fix inorganic carbon during C3 photosynthesis. Using qPCR primers that were designed comprehensively against all known zooxanthellal form II RuBisCO gene sequences (rbcII) in existing databases, we demonstrated that the outer mantle of T. squamosa (TS) had the greatest phototrophic potential as reflected by its high zooxanthellal rbcII (TSZrbcII) transcript level, which varied among different regions of the outer mantle. The other four organs also expressed moderate levels of TSZrbcII, despite the lack of iridophores and direct light exposure. Importantly, light exposure led to significant increases in the protein abundance of TSZRBCII in the outer mantle but not the other four organs. Taken together, these results indicate that organs inside the mantle cavity had low phototrophic potentials, but zooxanthellae residing inside these organs might serve some unidentified functions to benefit the host.en
dc.description.urihttps://doi.org/10.1007/s00338-020-01898-7-
dc.language.isoenen
dc.subjectCoral reefsen
dc.subjectLight-enhanced calcificationen
dc.subjectPhotosynthesisen
dc.subjectSymbiosisen
dc.subjectZooxanthellaeen
dc.titlePhototrophic potential and form II ribulose-1,5-bisphosphate carboxylase/oxygenase expression in five organs of the fluted giant clam, Tridacna squamosaen
dc.typePostprinten
dc.identifier.doi10.1007/s00338-020-01898-7-
dc.grant.idR-154-000-A37-114-
dc.grant.idR‐154‐000‐B69-114-
dc.grant.fundingagencyMinistry of Education, Singapore-
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