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Chan, J. W. J., Boo, M. V., Wong, W. P., Chew, S. F., & Ip, Y. K. (2021). Illumination enhances the protein abundance of sarcoplasmic reticulum Ca2+-ATPases-like transporter in the ctenidium and whitish inner mantle of the giant clam, Tridacna squamosa, to augment exogenous Ca2+ uptake and shell formation, respectively. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 251, Article 110811. Advance online publication https://doi.org/10.1016/j.cbpa.2020.110811
The fluted giant clam, Tridacna squamosa, can perform light-enhanced shell formation, aided by its symbiotic dinoflagellates (Symbiodinium, Cladocopium, Durusdinium), which are able to donate organic nutrients to the host. During light-enhanced shell formation, increased Ca2+transport from the hemolymph through the shell-facing epithelium of the inner mantle to the extrapallial fluid, where calcification occurs, is necessary. Additionally, there must be increased absorption of exogenous Ca2+from the surrounding seawater, across the epithelial cells of the ctenidium (gill) into the hemolymph, to supply sufficient Ca2+for light-enhanced shell formation. When Ca2+moves across these epithelial cells, the low intracellular Ca2+concentration must be maintained. Sarco (endo)plasmic reticulum Ca2+-ATPase (SERCA) regulates the intracellular Ca2+concentration by pumping Ca2+into the sarcoplasmic/endoplasmic reticulum (SR/ER) and Golgi apparatus. Indeed, the ctenidium and inner mantle of T. squamosa, expressed a homolog of SERCA (SERCA-like transporter) that consists of 3009 bp, encoding 1002 amino acids of 110.6 kDa. SERCA-like-immunolabeling was non-uniform in the cytoplasm of epithelial cells of ctenidial filaments, and that of the shell-facing epithelial cells of the inner mantle. Importantly, the protein abundance of SERCA-like increased significantly in the ctenidium and the inner mantle of T. squamosa after 12 h and 6 h, respectively, of light exposure. This would increase the capacity of pumping Ca2+into the endoplasmic reticulum and avert a possible surge in the cytosolic Ca2+concentration in epithelial cells of the ctenidial filaments during light-enhanced Ca2+absorption, and in cells of the shell-facing epithelium of the inner mantle during light-enhanced shell formation.
This is the original draft, prior to peer-review, of a manuscript published in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. The published version is available online at https://doi.org/10.1016/j.cbpa.2020.110811
Grant no. R-154-000-A37-114
Grant no. R-154-000-B69-114
Ministry of Education, Singapore
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checked on Mar 9, 2021
checked on Mar 9, 2021
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