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Electrochemical sensing based on sol-gel electrodes
Author
Yeo, Woei Ter
Supervisor
Tan, Swee Ngin
Abstract
In this study, the sol-gel technique has been used primarily for the development of electrochemical sensor and biosensor for various sensing applications. The target analytes have clinical and environmental significance.
The first part of this study focuses on the development of a selective mediated electrochemical biosensor for the determination of H2O2. A combination of enzyme catalysis and amperometric measurements has been employed as the system for the analysis. Peroxidase, a highly stable, efficient and powerful catalyst, is one of the enzymes that show excellent selectivity for its substrate, H2O2. Horseradish peroxidase (HRP) has been exploited in this study for the fabrication of a sol-gel modified HRP biosensor. The optimisation fabrication and response characteristics of the H2O2 biosensor have been evaluated. The selectivity of the biosensor against interferences has been studied.
The second part of this study focuses on the development of a sol-gel carbon composite electrode (CCE) for heavy metal sensing application. Carbon nanotubes, a new family of nanomaterials, has been used to modify the surface of a sol-gel CCE to fabricate a new chemically modified electrode (CME). This CME has been used for the detection of trace amount of aqueous Cd2+ and Pb2+ based on anodic stripping voltammetry (ASV). The optimisation fabrication and response characteristics of this CME have been evaluated and its applicability assessed in a water sample analysis.
The first part of this study focuses on the development of a selective mediated electrochemical biosensor for the determination of H2O2. A combination of enzyme catalysis and amperometric measurements has been employed as the system for the analysis. Peroxidase, a highly stable, efficient and powerful catalyst, is one of the enzymes that show excellent selectivity for its substrate, H2O2. Horseradish peroxidase (HRP) has been exploited in this study for the fabrication of a sol-gel modified HRP biosensor. The optimisation fabrication and response characteristics of the H2O2 biosensor have been evaluated. The selectivity of the biosensor against interferences has been studied.
The second part of this study focuses on the development of a sol-gel carbon composite electrode (CCE) for heavy metal sensing application. Carbon nanotubes, a new family of nanomaterials, has been used to modify the surface of a sol-gel CCE to fabricate a new chemically modified electrode (CME). This CME has been used for the detection of trace amount of aqueous Cd2+ and Pb2+ based on anodic stripping voltammetry (ASV). The optimisation fabrication and response characteristics of this CME have been evaluated and its applicability assessed in a water sample analysis.
Date Issued
2008
Call Number
QD115 Yeo
Date Submitted
2008