Options
Efficient green synthesis of biocompatible MPN fluorescent microspheres via hydrophobic-force-driven strategy for enhanced immunochromatographic assays
Loading...
Type
Article
Citation
Lai, X., Huang, S., Zhang, G., Ang, E. H., Yuan, H., Su, L., Liu, C., Deng, S., & Lai, W. (2024). Efficient green synthesis of biocompatible MPN fluorescent microspheres via hydrophobic-force-driven strategy for enhanced immunochromatographic assays. Journal of Hazardous Materials, 480, Article 136390. https://doi.org/10.1016/j.jhazmat.2024.136390
Author
Lai, Xiaocui
•
Huang, Shijin
•
Zhang, Ganggang
•
•
Yuan, Hongxin
•
Su, Liu
•
Liu, Cong
•
Deng, Shengliang
•
Lai, Weihua
Abstract
The unique fluorescence properties of aggregation-induced emission (AIE) fluorescent microspheres (FMs) make them ideal signal markers. Traditional synthesis methods are complex, labor-intensive, and hazardous, leading to AIEFMs that lack biocompatibility and require further modification for immunoprobe preparation. This study introduces a novel hydrophobic force-driven method for rapid synthesis of highly biocompatible FMs (H-FMs), demonstrating their benefits in immunochromatographic assay (ICA) applications. The metal-polyphenol network (MPN) shell around the AIEgen core structure of H-FMs is quickly and safely formed by depositing MPN onto AIEgen nano-aggregates, achieving high dye utilization, affordability, and design flexibility, while producing H-FMs with fluorescence across 300–800 nm. The excellent biocompatibility of H-FMs eliminates the need for additional modifications, allowing antibodies to be coupled swiftly (within 10 min) with a high coupling efficiency of 93.4 %. The resulting immunoprobes exhibit strong target recognition and 90.6 % fluorescence retention over 30 days. These features support their application in double antibody sandwich and competitive ICA formats, with detection limits of 9.62 × 10² CFU/mL for E. coli O157:H7 and 0.0081 ng/mL for AFM1. This study provides new insights into designing fluorescent probes for safety monitoring of hazardous materials in the environment.
Date Issued
2024
Publisher
Elsevier
Journal
Journal of Hazardous Materials