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Polydopamine-modulated anisotropic co-growth plasmonic blackbody for efficient ultra-broad-spectrum quenching to establish multicolor fluorescent immunoassay
Citation
Lai, X., Zhang, G., Zhang, G., Su, L., Liu, C., He, W., Ang, E. H., Lai, W., & Deng, S. (2024). Polydopamine-modulated anisotropic co-growth plasmonic blackbody for efficient ultra-broad-spectrum quenching to establish multicolor fluorescent immunoassay. Chemical Engineering Journal, 495, Article 153083. https://doi.org/10.1016/j.cej.2024.153083
Author
Lai, Xiaocui
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Zhang, Ganggang
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Zhang, Gan
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Su, Liu
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Liu, Cong
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He, Weihua
•
•
Lai, Weihua
•
Deng, Shengliang
Abstract
Fluorescence quenching immunoassays have received increasing attention due to their high signal-to-noise ratio, among which multi-color fluorescence quenching methods have great advantages in detecting multiple targets. Preparing an efficient and broad-spectrum quencher is a prerequisite for establishing high-performance multicolor fluorescence quenching immunoassays. In this study, we propose a polydopamine (PDA) modulated seedless co-growth method to synthesize anisotropic plasmonic blackbody (APB) with highly branched chrysanthemum −like shape for efficient ultra-broad-spectrum quenching and utilize APB to establish a sensitive multicolor fluorescent immunoassay. The key to APB synthesis lies in the reduction of Au3+ by dopamine, which simultaneously undergoes oxidation and self-polymerization in situ to form a PDA film. This film continuously deposits on the Au crystal seeds, creating a robust end-capping effect and inducing continuous anisotropic growth of Au. The dual synergistic effects of PDA and the unique 3D-spatial structure contribute to APB’s robust ultra-broad-spectrum UV absorption (6.86 × 1011 L cm−1 mol−1) from 300 to 800 nm, along with efficient ultra-broad-spectrum fluorescence quenching ability (90.1 %). This quenching ability extends to various types of fluorescence microspheres covering the range of 300–800 nm with different excitation and emission spectra. Subsequently, we applied APB in the development of a multicolor fluorescence quenching immunochromatographic assay, enabling simultaneous detection of chloramphenicol (limit of detection, LOD = 0.0045 ng/mL) and sulfadimidine (LOD = 0.038 ng/mL) in milk. This research not only offers a novel direction for designing efficient broad-spectrum quenchers but also advances the practical application of fluorescence quenching sensors.
Publisher
Elsevier
Journal
Chemical Engineering Journal
Grant ID
32160605
82374031
20224BAB205039
2023YSBG10003
2022YJC2005
2023YJC2006
Funding Agency
National Natural Science Foundation of China
Natural Science Foundation of Jiangxi Province
Pilot Demonstration Project of Ceilings in Funding for Provincial Science and Technology Program of Jiangxi Academy of Sciences