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Anticancer properties of filamentous marine cyanobacteria in Singapore
Author
Chew, Geraldine Jia Zhen
Supervisor
Tan, Lik Tong
Lee, Peter Peng Foo
Abstract
Marine cyanobacteria have been known to produce an extensive array of toxic secondary metabolites and have been viewed as a great source of pharmaceuticals. Several potential marine cyanobacterial secondary metabolites have been isolated for clinical research in areas such as antimicrobial, anti-inflammatory and anticancer activities.
Six strains of filamentous marine cyanobacteria belonging to the genus Lyngbya were collected from the southern islands of Singapore, namely Pulau Hantu Kechil, Sentosa Rocky Shore, St John’s Island, North Semakau, Pulau Hantu Besar and Sentosa Cove. Crude organic cyanobacterial extracts were prepared from each strain and tested for proteasome inhibition activity and cytotoxicity through MTT assay.
Marine cyanobacterial extracts prepared from samples obtained from Sentosa Rocky Shore and Sentosa Cove displayed the greatest proteasome inhibition levels for all three proteasome catalytic sites when tested at 10 ppm, 100 ppm and 1000 ppm (Fig. 3.2, 3.3 and 3.4). Extracts prepared from cyanobacterial strains collected at Pulau Hantu Besar displayed potent cytotoxicity against MOLT-4 leukemia cells even at the low concentration of 10 ppm (Fig. 3.1). Marine cyanobacteria samples from these three sites present potential anticancer properties available for further analysis.
Six strains of filamentous marine cyanobacteria belonging to the genus Lyngbya were collected from the southern islands of Singapore, namely Pulau Hantu Kechil, Sentosa Rocky Shore, St John’s Island, North Semakau, Pulau Hantu Besar and Sentosa Cove. Crude organic cyanobacterial extracts were prepared from each strain and tested for proteasome inhibition activity and cytotoxicity through MTT assay.
Marine cyanobacterial extracts prepared from samples obtained from Sentosa Rocky Shore and Sentosa Cove displayed the greatest proteasome inhibition levels for all three proteasome catalytic sites when tested at 10 ppm, 100 ppm and 1000 ppm (Fig. 3.2, 3.3 and 3.4). Extracts prepared from cyanobacterial strains collected at Pulau Hantu Besar displayed potent cytotoxicity against MOLT-4 leukemia cells even at the low concentration of 10 ppm (Fig. 3.1). Marine cyanobacteria samples from these three sites present potential anticancer properties available for further analysis.
Date Issued
2014
Call Number
QR99.63 Che
Date Submitted
2014