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Analyses of plant hormones
Author
Peh, Yunn Chyn
Supervisor
Tan, Swee Ngin
Yong, J. W. H. (Jean W. H.)
Abstract
In tissue culture, fresh banana and commercial banana powder have been widely used as a crude source of cytokinins (CKs). Furthermore, banana extracts have been used in tissue culture as the special additive to promote cell or organ elongation. This role is typically attributed to the physiological roles of gibberellins (GAs).
Although there were various reports on the growth-promoting properties of banana additives, the identification and levels of CKs and other phytohormones in banana have not been properly characterized.
The overall aim of this study was to apply and develop suitable separation and purification techniques for the characterization of endogenous phytohormones present in banana. The characterization of CKs in banana will be crucial as this information would allow the tissue culture industry to deduce the uncharacterized components which are responsible for the excellent efficacy of banana in promoting growth and development of tissue cultured plantlets.
The first part of the project investigated the different CKs present in banana using various solid phase extraction (SPE) approaches for purification. Subsequently, the purified sample is analysed by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LCMS) techniques. Four CKs were found in banana, namely trans-zeatin Oglucoside (ZOG), trans-zeatin riboside (ZR), dihydrozeatin O-glucoside (DZOG) and trans-zeatin (Z). The recovery of cytokinins in banana was about 8% and this could be due to the complex nature of the matrix.
The second part of this project aimed to develop an efficient SPE method for the purification of GAs. This new SPE method developed would be crucial for the subsequently GAs identification and quantification to be used for the screening of GAs in banana and other plant materials, by linking it to a suitable analytical method, e.g. HPLC or CE. This novel SPE purification approach developed showed good recovery of 94% for GA3.
Although there were various reports on the growth-promoting properties of banana additives, the identification and levels of CKs and other phytohormones in banana have not been properly characterized.
The overall aim of this study was to apply and develop suitable separation and purification techniques for the characterization of endogenous phytohormones present in banana. The characterization of CKs in banana will be crucial as this information would allow the tissue culture industry to deduce the uncharacterized components which are responsible for the excellent efficacy of banana in promoting growth and development of tissue cultured plantlets.
The first part of the project investigated the different CKs present in banana using various solid phase extraction (SPE) approaches for purification. Subsequently, the purified sample is analysed by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LCMS) techniques. Four CKs were found in banana, namely trans-zeatin Oglucoside (ZOG), trans-zeatin riboside (ZR), dihydrozeatin O-glucoside (DZOG) and trans-zeatin (Z). The recovery of cytokinins in banana was about 8% and this could be due to the complex nature of the matrix.
The second part of this project aimed to develop an efficient SPE method for the purification of GAs. This new SPE method developed would be crucial for the subsequently GAs identification and quantification to be used for the screening of GAs in banana and other plant materials, by linking it to a suitable analytical method, e.g. HPLC or CE. This novel SPE purification approach developed showed good recovery of 94% for GA3.
Date Issued
2007
Call Number
QK898.H67 Peh
Date Submitted
2007