Drought does not induce crassulacean acid metabolism (CAM) but regulates photosynthesis and enhances nutritional quality of Mesembryanthemum crystallinum

Abstract

Physiology and nutritional quality of a facultative CAM plant <i>Mesembryanthemum crystallinum</i> under drought stress alone are poorly understood. To induce drought, <i>M. crystallinum</i> was cultured aeroponically with different nutrient spraying intervals such as 5, 30, 60 and 240 min. The long spraying interval such as 240 min resulted in lower mass of root and shoot, shorter total root length with less tips and smaller surface area, compared to short interval of 5 min. Grown under the longest spraying interval of 240 min, <i>M. crystallinumalso</i> had significantly higher leaf dry matter content but lower leaf succulence. However, <i>CAM acidity</i> was undetectable for any plants. Although <i>M. crystallinum</i> grown under extended spraying intervals had higher photosynthetic pigments, they utilized lesser light energy and did not dissipate heat as effectively as those grown under 5 min. Compare to other shorter spraying intervals, photosynthetic gas exchange rates were significantly reduced under 240 min spraying interval, indicating signs of water deficit stress. Shoot nitrate, total reduced nitrogen, total soluble protein and Rubisco concentrations were similar for all plants. For phytochemicals and dietary minerals, plants grown under 240 min spraying interval had significantly higher values than the other plants. Therefore, drought does not result in the <i>induction</i> of <i>CAM</i> but regulates photosynthetic performance and enhances nutritional quality of <i>M. crystallinum.</i>