Browsing by Author "Goh, Hui Chin"
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- PublicationOpen AccessBenderamide A, a cyclic depsipeptide from a Singapore collection of marine cyanobacterium cf. Lyngbya sp.(2018)
;Ding, Gary Chi Ying ;Ong, Marshall Ji Fa ;Goh, Hui Chin ;Coffill, Cynthia R.Benderamide A (1), a (S)-2,2-dimethyl-3-hydroxy-7-octynoic acid (S-Dhoya)-containing cyclic depsipeptide that belongs to the kulolide superfamily, was isolated from a Singapore collection of cf. Lyngbya sp. marine cyanobacterium using a bioassay-guided approach. While the planar structure of 1 was elucidated using a combination of 1D and 2D NMR experiments and MS analysis, the absolute configuration was subsequently achieved using the results obtained from Marfey’s analysis, comparative analysis of nuclear overhauser effect spectroscopy (NOESY) with the known compound 3, and one dimensional-nuclear overhauser effect (1D-NOE). Although 1 did not display antiproliferative activity against MCF7 breast cancer cells, the presence of an Ala instead of Gly suggests a possible mechanistic pathway to explain the consequential decrease in cytotoxicity compared to the closely related 2. In addition, results obtained from an LC–MS/MS-based molecular networking algorithm revealed two other closely related compounds encouraging further identification and isolation from the same marine cyanobacterium extract.WOS© Citations 6 240 50Scopus© Citations 8 - PublicationRestrictedDevelopment of the Singapore Diagnostic Reading Assessment: Primary 1(2008-06)
;Koh, Kim Hong ;Wang, Feifei ;Nhu, MichaelGoh, Hui Chin"This report describes the work undertaken by CRPP staff in the test development project entitled Development of Singapore Diagnostic Reading Assessment (Sing*Read Assessment) - Primary 1. The report focuses on the rationales for developing a new P1 diagnostic reading assessment for use with Singapore students and the empirical evidence of the technical quality of the five reading tests, which are embedded in the Sing*"-- [p. 1] 0f executive summary.277 68 - PublicationOpen AccessDraft genome sequence of Bacillus sp. strain 007/aia-02/001, isolated from the marine sponge Coelocarteria singaporensis(2019)
;Ong, Marshall Ji Fa ;Goh, Hui ChinWe report the draft genome sequence of a marine bacterium, Bacillus sp. strain 007/AIA-02/001, isolated from the marine sponge Coelocarteria singaporensis, obtained from water off the coast of Singapore. The analysis of the bacterial genome using the bioinformatics tool antiSMASH 4.0.2 showed the presence of a number of unique natural product biosynthetic pathways.WOS© Citations 1Scopus© Citations 1 100 65 - PublicationRestrictedExplaining exam anxiety in Singaporean students: the influence of family, pedagogical and individual characteristics(2008-03)
;Goh, Hui Chin ;Hogan, DavidWith increasing pressure for young people to excel academically, the issue of exam anxiety warrants much attention as it bears on the validity of the testing procedure. Using data from the CRPP Life Pathways Study, a survey of 2,514 Secondary 1 students from 39 schools, this paper investigates the influences of family, pedagogical and individual characteristics on cognitive exam anxiety (CEA) among Singapore students using hierarchical regression. Controlling for demographic factors, expectations explained some variance while family background and authentic pedagogy did not. Subjective wellbeing and problem-solving skills further accounted for a significant proportion of variance. It is suggested that the family and school settings could instead be playing an indirect role in minimizing CEA through promoting student wellbeing and problem-solving skills.394 78 - PublicationRestrictedGenomic approach to the biomedical potential and diversity of marine bacteria associated with marine samples from Singapore(2018)Goh, Hui ChinMarine sponges are one of the oldest metazoan that have survived for more than 600 million years. As such, they have the capability to adapt through the major changes in the ocean. They are also sessile filter feeders that have high efficiency in filtering water through the aquiferous system, harboring diverse microbial communities, such as actinobacteria, fungi and cyanobacteria. Actinomycetes in particular, resides within the sponge tissue and they are well known for the ability to produce a variety of secondary metabolites that are of great potential for the pharmaceutical industry. The main objective of the present study is to assess the biomedical potential as well as the bacterial diversity of deep water marine samples using culture- dependent method and genomic approaches. The study involved the initial mechanical dredging along the Straits of Singapore to collect marine sponges and sediments. Marine bacteria were then isolated from the deep- water samples using a variety of marine culture media. The isolated marine bacterial colonies were then cultured in marine broth, followed by the extraction of their gDNA using molecular kits. The 16S rRNA profile of the bacterial gDNA was assessed via PCR and Sanger sequencing. Based on the bioassay and molecular networking data obtained from another study, selected marine bacterial strains were prioritized for whole genome sequencing. The bacterial genome data, achieved via whole genome sequencing were then analyzed and annotated using anti SMASH, for the presence of novel natural products biosynthetic gene clusters. Overall, this study presents data related to the diversity of marine bacteria associated with deep water marine samples as well as the detection of potential novel natural products using bioinformatics tools.
148 16 - PublicationOpen AccessIntegrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore(2019)
;Ong, Marshall Ji Fa ;Goh, Hui Chin ;Lim, Swee Cheng ;Pang, Li Mei ;Chin, Joyce Seow Fong ;Tan, Koh Siang ;Liang, Zhao-Xun ;Yang, Liang ;Glukhov, Evgenia ;Gerwick, William H.With 70% of the Earth’s surface covered in water, the marine ecosystem offers immense opportunities for drug discovery and development. Due to the decreasing rate of novel natural product discovery from terrestrial sources in recent years, many researchers are beginning to look seaward for breakthroughs in new therapeutic agents. As part of an ongoing marine drug discovery programme in Singapore, an integrated approach of combining metabolomic and genomic techniques were initiated for uncovering novel anti-quorum sensing molecules from bacteria associated with subtidal samples collected in the Singapore Strait. Based on the culture-dependent method, a total of 102 marine bacteria strains were isolated and the identities of selected strains were established based on their 16S rRNA gene sequences. About 5% of the marine bacterial organic extracts showed quorum sensing inhibitory (QSI) activity in a dose-dependent manner based on the Pseudomonas aeruginosa QS reporter system. In addition, the extracts were subjected to mass spectrometry-based molecular networking and the genome of selected strains were analysed for known as well as new biosynthetic gene clusters. This study revealed that using integrated techniques, coupled with biological assays, can provide an effective and rapid prioritization of marine bacterial strains for downstream large-scale culturing for the purpose of isolation and structural elucidation of novel bioactive compounds.WOS© Citations 13Scopus© Citations 15 218 50 - PublicationOpen AccessInteraction between cognitive and non-cognitive factors: The influences of academic goal orientation and working memory on mathematical performance(Taylor & Francis, 2013)
;Lee, Kerry ;Ning, FloraGoh, Hui ChinAlthough the effects of achievement goals and working memory on academic performance are well established, it is not clear whether achievement goals and working memory jointly affect academic performance. Children from Primary 4 and 6 (N = 608) were administered (a) measures of working memory and updating from the Automated Working Memory Battery and a running span task, (b) performance and mastery goal measures from the Inventory of School Motivation, and (c) a battery of standardised and curriculum based mathematical tests. Structural equation modelling showed that performance goal had a negative effect on mathematics. Both mastery and performance goals had direct (positive and negative, Although the effects of achievement goals and working memory on academic performance are well established, it is not clear whether achievement goals and working memory jointly affect academic performance. Children from Primary 4 and 6 (N = 608) were administered (a) measures of working memory and updating from the Automated Working Memory Battery and a running span task, (b) performance and mastery goal measures from the Inventory of School Motivation, and (c) a battery of standardised and curriculum based mathematical tests. Structural equation modelling showed that performance goal had a negative effect on mathematics. Both mastery and performance goals had direct (positive and negative,WOS© Citations 26 126 621Scopus© Citations 36 - PublicationOpen AccessTrikoramide A, a prenylated cyanobactin from the marine cyanobacterium symploca hydnoides(2019)
;Phyo, Ma Yadanar ;Ding, Gary Chi Ying ;Goh, Hui Chin ;Goh, Jun Xian ;Ong, Ji Fa Marshall ;Chan, Siew Herng ;Yung, Pui Yi Maria ;Candra, HartonoA new cyclic decapeptide, trikoramide A (1), has been isolated from samples of the marine cyanobacterium Symploca hydnoides, collected from Bintan Island, Indonesia. Trikoramide A (1) is a C-prenylated cyclotryptophan-containing cyanobactin. Its planar structure was deduced by 1D and 2D NMR spectroscopy as well as HR–MS/MS data. In addition, its absolute configuration was determined by Marfey’s method and 2D NOESY NMR spectroscopic analysis. Compound 1 possessed cytotoxicity against the MOLT-4 and AML2 cancer cell lines with IC50 values of 4.8 and 8.2 μM, respectively.WOS© Citations 13 107 70Scopus© Citations 15 - PublicationRestrictedWorking memory, academic goal orientation, and Mathematics performance(2010)Goh, Hui ChinIn academic achievement, there has been a call for working memory and goals to be considered together (Covington, 2000). This study thus examines the way in which working memory, goal orientation and Mathematics achievement are related. Participants were 316 upper primary school students. Through a series of structural equation models, it was argued that the data best fit a model where goal orientation and working memory separately accounted for unique variance in Mathematics proficiency (additive model). Results are consistent with studies in cognitive psychology, and suggest that goals impact achievement not merely through the increased allocation of mental resources or effort to working memory. This study contends that rather than rate of learning, goals influence the quality of learning through learning strategies. Juxtaposing these findings with the levels of endorsement that local students place upon academic goals, pedagogical implications are discussed.
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